| dc.contributor.advisor | Olivero Verbel, Jesús | |
| dc.contributor.author | Franco Valencia, Karen | |
| dc.date.accessioned | 2022-03-22T15:36:44Z | |
| dc.date.available | 2022-03-22T15:36:44Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | La mucosa humana presenta diversas comunidades microbianas y ha sido estimado que más de 700 diferentes especies microbianas, son capaces de habitar en medio de interacciones sinérgicas, mutualistas y antagónicas entre los diferentes microorganismos, contribuyendo así al desarrollo y acumulación de comunidades polimicrobianas que forman una biopelícula denominada placa dental. Cuando el equilibrio microbiano es afectado, suceden en la biopelícula alteraciones que conllevan a enfermedades tales como la gingivitis, la periodontitis y la caries, las cuales afectan un gran número de personas. La caries dental es una enfermedad microbiana que sigue constituyéndo un problema de salud en todo el mundo, afectando mayoritariamente a la población adulta y escolar entre el 60% y 90% según la OMS en el 2007. Por lo tanto, dentro del amplio espectro de bacterias de la microbiota oral, el género Streptococcus spp. es el más importante y prevalente, siendo la especie mutans el principal agente etiológico relacionado con la caries. Por lo anterior, el objetivo de este estudio fue determinar la actividad antibacteriana de un enjuague bucal natural a base de los aceites esenciales de Lippia origanoides y Mentha piperita evaluando su aceptación organoléptica en un grupo de donantes voluntarios, analizando su citotoxicidad (ensayo MTT) y genotoxicidad (ensayo cometa) en cultivos in vitro de células HepG2 y evaluando su acción microbiológica. Posteriormente, fue evaluada la actividad antimicrobiana y comparada con un enjuague bucal comercial, además de valorar de la concentración mínima inhibitoria (CMI) frente a cepas ATCC de Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae y Pseudomona aeruginosa. El enjuague bucal presentó una efectiva aceptación organoléptica. Los resultados obtenidos en la citotoxicidad (ensayo MTT) demuestran que a bajas y altas concentraciones las células HepG2 pueden tolerar el aceite, para el caso de la genotoxicidad (ensayo cometa) las concentraciones más bajas sugieren una baja sensibilidad de las células HepG2 a L. origanoides. El enjuague bucal natural presenta actividad antibacteriana en las concentraciones de 5% y 10%. El método utilizado para valorar la concentración mínima inhibitoria (CMI) fue el de microdilución en caldo con placas de 96 pozos. Las cepas de S. mutans y S. aureus tuvieron un grado de inhibición a concentraciones de 50 mg/mL, 100 mg/mL y 150 mg/ mL, a diferencia de las cepas de K. pneumoniae y P. aeruginosa, en las que no hubo actividad inhibitoria. | spa |
| dc.description.degreelevel | Maestría | spa |
| dc.description.degreename | Magíster en Microbiología | spa |
| dc.format.mimetype | application/pdf | spa |
| dc.identifier.uri | https://hdl.handle.net/11227/14828 | |
| dc.identifier.uri | http://dx.doi.org/10.57799/11227/1367 | |
| dc.language.iso | spa | spa |
| dc.publisher | Universidad de Cartagena | spa |
| dc.publisher.faculty | Facultad de Medicina | spa |
| dc.publisher.place | Cartagena de India | spa |
| dc.publisher.program | Maestría en Microbiología | spa |
| dc.rights | Derechos Reservados - Universidad de Cartagena, 2013 | spa |
| dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
| dc.rights.creativecommons | Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) | spa |
| dc.subject.armarc | Microbiología | |
| dc.subject.armarc | Odontología preventiva | |
| dc.subject.armarc | Caries dentales - Prevención | |
| dc.subject.armarc | Dientes - Enfermedades | |
| dc.title | Actividad antibacteriana del enjuague bucal a base de lippia origanoides y mentha piperita frente a la flora oral de estudiantes de la Universidad de Cartagena | spa |
| dc.type | Trabajo de grado - Maestría | spa |
| dc.type.coar | http://purl.org/coar/resource_type/c_bdcc | spa |
| dc.type.content | Text | spa |
| dc.type.driver | info:eu-repo/semantics/masterThesis | spa |
| dc.type.redcol | https://purl.org/redcol/resource_type/TM | spa |
| dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
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| dspace.entity.type | Publication | |
| oaire.accessrights | http://purl.org/coar/access_right/c_abf2 | spa |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
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