| dc.contributor.advisor | Yasnot Acosta, María Fernanda (Director/a) | |
| dc.contributor.advisor | Venegas Hermosilla, Juan (Asesor/a) | |
| dc.contributor.author | Orjuela González, Lorena Isabel | |
| dc.date.accessioned | 2020-11-24T15:24:16Z | |
| dc.date.available | 2020-11-24T15:24:16Z | |
| dc.date.issued | 2019 | |
| dc.description | Tesis (Doctorado en Medicina Tropical). -- Universidad de Cartagena. Facultad de Medicina, Doctorado Medicina Tropical, 2019 | es |
| dc.description.abstract | Se realizaron evaluaciones de resistencia a insecticidas en los tres principales vectores de malaria en Colombia, An. albimanus, An. nuneztovari s.l. y An. darlingi en 11 localidades de los departamentos de Antioquia, Chocó, Córdoba, Valle del Cauca y Norte de Santander. Las evaluaciones de resistencia se hicieron siguiendo la metodología de botellas impregnadas propuesta por el CDC. En cada una de las localidades se expusieron mínimo 100 mosquitos a las concentraciones diagnóstico de cada uno de los insecticidas. Los insecticidas evaluados fueron los piretroides: deltametrina (12,5 µg/ml), lambdacialotrina (12,5 µg/ml), alfacipermetrina (12,5 µg/ml), permetrina (21,5 µg/ml) y el organoclorado DDT (100ug/ml). Los resultados fueron analizados teniendo en cuenta los criterios de mortalidad propuestos por los CDC y de acuerdo con estos criterios, An. darlingi de las localidades de Bocas de Pune, Tagachí y Encharcazón en Chocó, An. nuneztovari s.l. de las localidades de Santa Rosa en Norte de Santander y Córdoba en Valle del Cauca y An. albimanus de la localidad de Panguí en Chocó evidenciaron resultados de resistencia y posible resistencia a insecticidas piretorides y DDT. En las localidades donde se evidenció resistencia, se realizaron evaluaciones de intensidad de la resistencia usando la metodología de botellas impregnadas propuesta por el CDC y se evidenció que An. darlingi de Bocas de Puné y Tagachi en Chocó, An. nuneztovari s.l de Córdoba en Valle del Cauca y An. albimanus de Panguí en Chocó presentaban una intensidad de resistencia baja. Debido a que en An. darlingi de Tagachí, Encharcazón y Bocas de Pune, en An. nuneztovari s.l. de Córdoba y Santa Rosa y en An. albimanus de Panguí se evidenció resistencia a piretroides y/o DDT y teniendo en cuenta que estas dos clases de insecticidas comparten el mismo sitio de acción, el canal de sodio dependiente de voltaje, se amplificó y secuenció el fragmento del gen que codifica para el segmento seis del dominio dos (S6II) del canal de sodio dependiente de voltaje en 130 muestras de An. albimanus, An. darlingi y An. nuneztovari s.l. 62 mosquitos sobrevivientes a las dosis y tiempo diagnóstico y 68 mosquitos muertos a la dosis y tiempo diagnóstico de alfacipermetrina, deltametrina, lambdacialotrina, permetrina y DDT, para identificar si existían mutaciones en los codones 1010, 1013 y 1014 en donde previamente se han reportado mutaciones asociadas con la resistencia a piretroides y DDT en otros vectores de malaria en el mundo y no se detectaron mutaciones, lo que sugiere que otros mecanismos de resistencia diferentes a kdr están involucrados en la baja intensidad de resistencia a insecticidas piretroides y DDT detectada en los tres principales vectores de malaria en Colombia. | es |
| dc.format.medium | application/pdf | es |
| dc.identifier.citation | TD615.532 / O49 | es |
| dc.identifier.uri | https://hdl.handle.net/11227/11219 | |
| dc.identifier.uri | http://dx.doi.org/10.57799/11227/148 | |
| dc.language.iso | spa | es |
| dc.publisher | Universidad de Cartagena | es |
| dc.rights.access | openAccess | es |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0 | es |
| dc.subject | Malaria - Investigaciones | es |
| dc.subject | Medicina alternativa | es |
| dc.subject | Enfermedades - Tratamiento | es |
| dc.subject | Insecticidas - Residuos | es |
| dc.subject | Medicina tropical | es |
| dc.title | Evaluación de la resistencia a los insecticidas piretroides y DDT en los principales vectores de malaria de Colombia y su asociación con mutaciones en el canal de sodio dependiente de voltaje | es |
| dc.type | Trabajo de grado - Doctorado | spa |
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Colombia, Bolívar, Cartagena
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