dc.contributor.advisor | Zakzuk Sierra, Josefina (Profesor(a) / Docente / Tutor(a)) | |
dc.contributor.author | Peñaranda Garcia, Daniel | |
dc.date.accessioned | 2019-02-25T21:02:35Z | |
dc.date.available | 2019-02-25T21:02:35Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | TM616.96 / P313 | es |
dc.identifier.uri | https://hdl.handle.net/11227/7379 | |
dc.identifier.uri | http://dx.doi.org/10.57799/11227/1341 | |
dc.description | Thesis (Magister in Immunology) - University of Cartagena. Institute of Immunological Research. Master's Degree in Immunology, 2018 | es |
dc.description.abstract | Background: Helminth infections can modify the immune response of the host and exert risk and protective effects on allergic diseases depending on the chronicity and intensity of
the infection. Blood populations such as eosinophils and regulatory B cells have been independently associated with helminth infection and allergic diseases, with eosinophils being used as biomarkers for severity. However, the effects of helminth infections in these
blood populations, and the predictive role of eosinophils in asthma severity is unclear in humans from endemic settings.
Objectives: To characterize the effects of Ascaris lumbricoides infection in eosinophils, regulatory B cells and asthma severity in asthmatic patients from a helminth endemic population.
Methods: In a rural community from the Colombia tropics, asthmatic patients were recruited and evaluated with a clinical questionnaire, specific and total IgE, complete blood
counts, skin prick test, serial coprologicals and spirometry. Regulatory B cell subsets were evaluated in a subset of asthmatic patients and controls from the community.
Results: A total of 82 patients with wheezing symptoms in the last year were recruited.
Complete blood counts showed a mean eosinophil count of 654 (±SD 634) cells/uL, with 48,7% (n=40) of patients having eosinophils greater than 400 cells/uL. The frequency of
active A.lumbricoides and T.trichiura infection were 46,3% (n=38) and 18,2% (n=15)
respectively. In the multivariate analysis, A.lumbricoides sensitization was associated with a 6.9-fold increase in the odds of having eosinophils greater than 400 cells/uL (p=0.002) and
House Dust Mite (HDM) sensitization was also associated with a 5,4-fold increase (p=0.008).
Helminth infection was associated with higher log-transformed eosinophils independently of age and HDM sensitization.We found lower percentage of CD24+CD38+ B cells in
asthmatic patients compared to control subjects and no significant differences between
infected and uninfected patients for CD1dhighCD5+ and CD24highCD38high B cell subsets.
Conclusion: In a rural community from the Colombian tropics, where helminths are endemic, sensitization to A.lumbricoides was more predictive of eosinophilia than
sensitization to house dust mites. The frequency of regulatory B cell subsets was lower in asthmatic patients than in controls. Further research should evaluate larger cohorts and
determine the basal level of eosinophils in this population and focus on the cellular function of regulatory B cells. | es |
dc.format.medium | application/pdf | |
dc.language.iso | spa | es |
dc.publisher | Universidad de Cartagena | es |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0 | |
dc.subject | Gusanos parásitos e intestinales | es |
dc.subject | Áscaris | es |
dc.subject | Parasitología médica | es |
dc.subject | Parasitismo | es |
dc.title | Effects of Ascaris lumbricoides in eosinophils, regulatory B cells and asthma severity in asthmatic patients from a helminth endemic population | es |
dc.type | Trabajo de grado - Maestría | spa |
dcterms.references | Starr MC, Montgomery SP. Soil-transmitted Helminthiasis in the United States: a systematic review--1940-2010. Am J Trop Med Hyg. 2011;85(4):680-684. doi:10.4269/ajtmh.2011.11-
0214. | |
dcterms.references | Pullan RL, Smith JL, Jasrasaria R, Brooker SJ. Global numbers of infection and disease burden of soil transmitted helminth infections in 2010. Parasit Vectors. 2014;7(1):37.
doi:10.1186/1756-3305-7-37. | |
dcterms.references | Hotez PJ, Brindley PJ, Bethony JM, King CH, Pearce EJ, Jacobson J. Helminth infections: the great neglected tropical diseases. J Clin Invest. 2008;118(4):1311-1321. doi:10.1172/JCI34261. | |
dcterms.references | Fumagalli M, Pozzoli U, Cagliani R, et al. Parasites represent a major selective force for interleukin genes and shape the genetic predisposition to autoimmune conditions. J Exp Med. 2009;206(6):1395-1408. doi:10.1084/jem.20082779. | |
dcterms.references | Chico ME, Vaca MG, Rodriguez A, Cooper PJ. Soil-transmitted helminth parasites and allergy: observations from Ecuador. Parasite Immunol. 2018:e12590. doi:10.1111/pim.12590. | |
dcterms.references | Wammes LJ, Mpairwe H, Elliott AM, Yazdanbakhsh M. Helminth therapy or elimination: Epidemiological, immunological, and clinical considerations. Lancet Infect Dis.
2014;14(11):1150-1162. doi:10.1016/S1473-3099(14)70771-6. | |
dcterms.references | Zakzuk J. Inmunorregulación inducida por helmintos: una actualización. IATREIA. 2016;29(2):182-193. doi:10.17533/udea.iatreia.v29n2a07. | |
dcterms.references | Caraballo L, Acevedo N, Zakzuk J. Ascariasis as a model to study the helminth/allergy relationships. Parasite Immunol. November 2018:e12595. doi:10.1111/pim.12595. | |
dcterms.references | Caraballo L, Zakzuk J, Lee BW, et al. Particularities of allergy in the Tropics. World Allergy Organ J. 2016;9(1):20. doi:10.1186/s40413-016-0110-7. | |
dcterms.references | Caraballo L. The tropics, helminth infections and hygiene hypotheses. Expert Rev Clin Immunol. 2018;14(2):99-102. doi:10.1080/1744666X.2018.1424543. | |
dcterms.references | Lambrecht BN, Hammad H. The immunology of asthma. Nat Immunol. 2015;16(1):45-56. doi:10.1038/ni.3049. | |
dcterms.references | McCracken JL, Tripple JW, Calhoun WJ. Biologic therapy in the management of asthma. Curr Opin Allergy Clin Immunol. 2016;16(4):375-382. doi:10.1097/ACI.0000000000000284. | |
dcterms.references | Walsh GM. Mepolizumab-based therapy in asthma. Curr Opin Allergy Clin Immunol. 2015;15(4):392-396. doi:10.1097/ACI.0000000000000183. | |
dcterms.references | Palomares O, Akdis M, Martín-Fontecha M, Akdis CA. Mechanisms of immune regulation in allergic diseases: the role of regulatory T and B cells. Immunol Rev. 2017;278(1):219-236.
doi:10.1111/imr.12555. | |
dcterms.references | van de Veen W, Stanic B, Wirz OF, Jansen K, Globinska A, Akdis M. Role of regulatory B cells in immune tolerance to allergens and beyond. J Allergy Clin Immunol. 2016;138(3):654-665.
doi:10.1016/j.jaci.2016.07.006. | |
dcterms.references | van der Vlugt LEPM, Mlejnek E, Ozir-Fazalalikhan A, et al. CD24(hi)CD27(+) B cells from patients with allergic asthma have impaired regulatory activity in response to
lipopolysaccharide. Clin Exp Allergy. 2014;44(4):517-528. doi:10.1111/cea.12238 | |
dcterms.references | van der Vlugt LEPM, Labuda LA, Ozir-Fazalalikhan A, et al. Schistosomes Induce Regulatory
Features in Human and Mouse CD1dhi B Cells: Inhibition of Allergic Inflammation by IL-10 and Regulatory T Cells. Bozza PT, ed. PLoS One. 2012;7(2):e30883. doi:10.1371/journal.pone.0030883. | |
dcterms.references | Obieglo K, Schuijs MJ, Ozir-Fazalalikhan A, et al. Isolated Schistosoma mansoni eggs prevent allergic airway inflammation. Parasite Immunol. 2018;40(10):e12579. doi:10.1111/pim.12579. | |
dcterms.references | van der Vlugt LEPM, Obieglo K, Ozir-Fazalalikhan A, Sparwasser T, Haeberlein S, Smits HH.
Schistosome-induced pulmonary B cells inhibit allergic airway inflammation and display a reduced Th2-driving function. Int J Parasitol. 2017;47(9):545-554. doi:10.1016/j.ijpara.2017.02.002. | |
dcterms.references | Smits HH, Hammad H, van Nimwegen M, et al. Protective effect of Schistosoma mansoni infection on allergic airway inflammation depends on the intensity and chronicity of
infection. J Allergy Clin Immunol. 2007;120(4):932-940. doi:10.1016/j.jaci.2007.06.009. | |
dcterms.references | van der Vlugt LEPM, Labuda LA, Ozir-Fazalalikhan A, et al. Schistosomes Induce Regulatory Features in Human and Mouse CD1dhi B Cells: Inhibition of Allergic Inflammation by IL-10 and Regulatory T Cells. Bozza PT, ed. PLoS One. 2012;7(2):e30883. doi:10.1371/journal.pone.0030883. | |
dcterms.references | Smits HH, Hammad H, van Nimwegen M, et al. Protective effect of Schistosoma mansoni infection on allergic airway inflammation depends on the intensity and chronicity of
infection. J Allergy Clin Immunol. 2007;120(4):932-940. doi:10.1016/j.jaci.2007.06.009. | |
dcterms.references | Zakzuk J, Casadiego S, Mercado A, Alvis-Guzman N, Caraballo L. Ascaris lumbricoides infection induces both, reduction and increase of asthma symptoms in a rural community.
Acta Trop. 2018;187:1-4. doi:10.1016/j.actatropica.2018.07.016. | |
dcterms.references | Dennis RJ, Caraballo L, García E, et al. Prevalence of asthma and other allergic conditions in Colombia 2009–2010: a cross-sectional study. BMC Pulm Med. 2012;12(1):17.
doi:10.1186/1471-2466-12-17. | |
dcterms.references | Miller MR, Hankinson J, Brusasco V, et al. Standardisation of spirometry. Eur Respir J. 2005;26(2):319-338. doi:10.1183/09031936.05.00034805. | |
dcterms.references | World Health Organization. Haemoglobin Concentrations for the Diagnosis of Anaemia and Assessment of Severity. https://www.who.int/vmnis/indicators/haemoglobin.pdf. Accessed November 27, 2018. | |
dcterms.references | Price DB, Rigazio A, Campbell JD, et al. Blood eosinophil count and prospective annual asthma disease burden: A UK cohort study. Lancet Respir Med. 2015;3(11):849-858.
doi:10.1016/S2213-2600(15)00367-7. | |
dcterms.references | Zeiger RS, Schatz M, Li Q, Chen W, Khatry DB. High Blood Eosinophil Count Is a Risk Factor for Future Asthma Exacerbations in Adult Persistent Asthma. J Allergy Clin Immunol Pract. 2014;2(6):741-750.e4. doi:10.1016/j.jaip.2014.06.005. | |
dcterms.references | Zeiger RS, Schatz M, Dalal AA, et al. Blood Eosinophil Count and Outcomes in Severe Uncontrolled Asthma: A Prospective Study. J Allergy Clin Immunol Pract. 2017;5(1):144-
153.e8. doi:10.1016/j.jaip.2016.07.015. | |
dcterms.references | Huang L, Appleton JA. Eosinophils in Helminth Infection: Defenders and Dupes. Trends Parasitol. 2016;32(10):798-807. doi:10.1016/j.pt.2016.05.004. | |
dcterms.references | Klion AD, Nutman TB. The role of eosinophils in host defense against helminth parasites. J Allergy Clin Immunol. 2004;113(1):30-37. doi:10.1016/j.jaci.2003.10.050 | |
dcterms.references | Alcântara-Neves NM, de S G Britto G, Veiga RV, et al. Effects of helminth co-infections on atopy, asthma and cytokine production in children living in a poor urban area in Latin
America. BMC Res Notes. 2014;7:817. doi:10.1186/1756-0500-7-817. | |
dcterms.references | O’Sullivan JA, Bochner BS. Eosinophils and eosinophil-associated diseases: An update. J Allergy Clin Immunol. 2017:1-13. doi:10.1016/j.jaci.2017.09.022. | |
dcterms.references | Rosenberg HF, Dyer KD, Foster PS. Eosinophils: Changing perspectives in health and disease. Nat Rev Immunol. 2013;13(1):9-22. doi:10.1038/nri3341. | |
dcterms.references | Buendía E, Zakzuk J, Mercado D, Alvarez A, Caraballo L. The IgE response to Ascaris molecular components is associated with clinical indicators of asthma severity. World Allergy Organ J. 2015;8(1):1-11. doi:10.1186/s40413-015-0058-z. | |
dcterms.references | Ahumada V, García E, Dennis R, et al. IgE responses to Ascaris and mite tropomyosins are risk factors for asthma. Clin Exp Allergy. 2015;45(7):1189-1200. doi:10.1111/cea.12513 | |
dcterms.references | van der Vlugt LEPM, Mlejnek E, Ozir-Fazalalikhan A, et al. CD24 hi CD27 + B cells from patients with allergic asthma have impaired regulatory activity in response to lipopolysaccharide. Clin Exp Allergy. 2014;44(4):517-528. doi:10.1111/cea.12238. | |
dcterms.references | Blair PA, Noreña LY, Flores-Borja F, et al. CD19+CD24hiCD38hi B Cells Exhibit Regulatory als but Are Functionally Impaired in Systemic Lupus Erythematosus Patients. Immunity. 2010;32(1):129-140. doi:10.1016/j.immuni.2009.11.009. | |
dcterms.references | Van Der Vlugt LEPM, Zinsou JF, Ozir-Fazalalikhan A, et al. Interleukin 10 (IL-10)-producing CD1dhi regulatory B cells from Schistosoma haematobium-infected individuals induce IL-10-
positive T cells and suppress effector T-cell cytokines. J Infect Dis. 2014;210(8):1207-1216. doi:10.1093/infdis/jiu257. | |
dc.rights.access | openAccess | |